Abstract: In the light of the special requirement of image mosaic of Data Matrix barcode which marked on cylindrical surface of tool,a new mosaic method is presented based on the feature of Data Matrix barcode image to remedy the shortages of current image mosaic algorithms.In our method,an image collection device with a gap is used to gather the sequence barcode images by rotating the tool which is put in the gap,then these images are corrected to eliminate the longitudinal displacement and divided into barcode modules,after that the algorithms based on minimum Euclidean distance and normalized cross-correlation are used to achieve images registration.In order to keep the barcode structure right and to avoid the failure caused by non-overlap images,these sequence images are fused into three parts at first according to the matching degree,then these three parts are corrected to eliminate the distortion and finally fused into a right size image according to modules.Our method can obtain the barcode position and the module division at the same time.The decoding rate of the method achieves 96% and the mosaic time is less than 250 ms in experiment.