Abstract: Aiming at the problem that honeycomb structure is fragile under concentrated load in the in-plane direction, a design method of non-uniform honeycomb structures is proposed using topology optimization density mapping. The method mapped the density information from topology optimization results to the wall thickness of honeycomb cells. Then a honeycomb structure with non-uniform wall thickness is obtained through optimizing the honeycomb cell size and density mapping parameters with response surface method and collaborative optimization strategy. A test-piece of thenon-uniform honeycomb is fabricated by 3D printing technology and tested under three-point bending, compared with an equivoluminal uniform honeycomb. It is validated that the proposed method could generate a non-uniform honeycomb structure with higher local load-bearing capacity.